The catalase lab essay

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The catalase lab essay

Metabolism The major objective of this experiment was to observe the effects of catalase under varying controlled conditions. The scope of this experiment includes Metabolic processes, such as cellular respiration, and it poisonous byproduct hydrogen peroxide.

The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time.

We The catalase lab essay write a custom essay sample on Enzyme Lab Report or any similar topic specifically for you Do Not Waste HIRE WRITER The major conclusion was that catalase reacts faster in warm temperatures that are neither freezing nor boiling, catalase performs well in lower concentrations than the substrate, and catalase prefers neutral pH levels around 7.

Introduction Enzymes are proteins that catalyze metabolic reactions vital for the survival and functioning of cells [1]. Without enzymes, metabolic processes would occur at unfeasible rates. Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration.

I asked if enzyme activity was affected when exposed to different conditions, such as temperature, substrate concentration, and pH levels.

My first hypothesis is that higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes. My second hypothesis is that smaller concentrations of enzyme in the enzyme to substrate concentration ratios produce higher reactions.

The catalase lab essay

The last hypothesis is that enzyme reactions work best at a neutral pH. I observed and recorded the effect of these conditions on catalase. The enzyme catalase is important because it handles the decomposition of approximately half of generated H2O2 in living organisms [2]. It is important for biologists to understand catalase because all cells produce hydrogen peroxide, and its the job of catalase to break it down.

This research will help increase the knowledge of enzyme activity in complex environments, and under what conditions the enzyme catalase will perform best.

The materials used in this experiment included 4 test tubes 5 for pHatalase, hydrogen peroxide, an ice water bath, a warm water bath, a boiling water bath, thermometer, test tube rack, a wax pencil, a metric ruler, a timing device, and pH solutions at 1, 4, 7, 10 and Three different experiments were tested in this lab; temperature, substrate concentration and pH.

The positive control of this experiment was the test tube kept in room temperature, and the negative control was the test tube placed in the ice. The independent variables of this experiment were time and temperature, and the dependent variable was the amount of reaction that occurred.

Each test tube was left at the designated temperature for two minutes, and then afterwards were tested every ten seconds for a minute. My technique included swirling the test tube 3 times each after every ten seconds, and then I would record the bubble height in centimeters.

In the Substrate concentration experiment, I filled the test tubes with different enzyme to hydrogen peroxide concentrations, which were measured in centimeters. Next, I labeled the test tubes accordingly; 1 1 cm: The positive control of the experiment was the test tube with the 1: The independent variables were time and the ratio of enzyme to substrate concentration and the dependent variable was the amount of reaction that occurred.

Each test tube was left at room temperature and then tested every ten seconds for a minute. The technique used was the same as experiment one. In the pH experiment, I filled all 5 of the beakers with 2 centimeters of Catalase and 3 centimeters of hydrogen peroxide.

The positive control was the pH 4 test tube, and the negative control was the pH 13 test tube. The independent variables were time and pH levels, and the dependent variable was the amount of eaction that occurred. Each test tube was left at room temperature for one minute and then tested every five seconds for thirty seconds.

The technique used was also the same as the first experiment. This supports my hypothesis that higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes.

The lowest reaction was the 4 to 1 ratio, this is because the 3 to 2 and 4 to 1 ratios, too much catalase was added. The catalase broke down all the hydrogen peroxide and then had a surplus after the reaction occurred.

This supports my hypothesis that smaller concentrations of enzyme in the enzyme to substrate concentration ratios produce higher reactions. The least reaction occurred at a pH of 13 because it is too basic for catalase, and it works independently from basic pH levels.

This supports my hypothesis that enzyme reactions work best at a neutral pH of 7. Discussion Principles, Relationships, and Generalizations. The principles of this experiment were that 1 higher temperatures amplify catalyzation, although too high of a temperature denatures enzymes, 2 smaller concentrations of enzyme in the enzyme to substrate concentration atios produce higher reactions, and 3 enzyme reactions work best at a neutral pH.

The correlation between these three principles is that they all speed up the process of enzyme activity. The basic generalization made is that enzymes are extremely efficient. Enzymes are biological catalysts that can speed up, and control, chemical reactions that would otherwise virtually never occur at normal body temperature.

Thousands of chemical reactions are occurring in the human body every moment of life, and each of these reactions is controlled by a particular enzyme [3]. Comparing Results Considering my experiments and data, my end result was the exact criterion I originally expected.Catalase is a naturally occurring enzyme that breaks down hydrogen peroxide into water and oxygen; it is essential to cellular respiration.

I asked if enzyme activity was affected when exposed to different conditions, such as temperature, substrate concentration, and pH levels.

Catalase Lab Essay Purpose The purpose of this catalase lab is to design simple experiments to demonstrate how various factors affect the rate of enzyme activity.

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Popular Topics How to Write a Summary of an Article? Catalase Lab Report Investigating the effect of different environmental factors on the reaction of liver and hydrogen peroxide Table of Contents 1 Design3 1.

This lab shows how the enzyme decomposes in hydrogen peroxide. - Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase.

Catalase lab report research paper.

Catalase lab ap biology essays

Quality custom essays ukc philippe bourgeois in search of respect essays xbox one vs ps4 controller comparison essay early greek philosophy and other essays on the great boekenweek essay super essay grading comments for teachers fieo international events essay. Catalase is present in most cells and high concentrations are found in liver and blood cells.

Essay on Enzyme Lab Report Words | 5 Pages Biology Lab: Catalase (Enzymes) Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. - Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis In essence, the main objective was to use chemical titration to measure and then calculate the rate of conversion of hydrogen peroxide (H2O2) to water and oxygen by using the enzyme catalase. catalase lab report Essay [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes .

Hydrogen peroxide is formed as a by-product of cellular respiration in cells but water is the most common one.

You were to analyze a variety of tests including analyzing a glucose solution for the presence of simple reducing sugars, analyzing a sample of vegetable oil for the presence of lipids, analyzing a ample of gelatin for the presence of protein, and analyzing an unknown sample to determine the presence of starch, simple reducing sugars, lipids, and proteins through chemical tests.

The catalase lab essay
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